Alpha Fetoprotein Test / Rapid AFP Test
Healthaw AFP Test kit is a rapid, direct binding test for the detection of Alpha Fetoprotein in serum.
INTENDED USE
Rapid AFP Test is an immunochromatography based one step in vitro test. It is designed for the rapid qualitative determination of AFP in serum or plasma specimens to aid in the diagnosis of Hepatocellular Carcinoma or fetal open neural tube defects..
PRINCIPLE OF THE ASSAY
Rapid AFP test is a sandwich immunoassay. When serum sample is added to sample pad, it moves through the conjugate pad and mobilizes gold-mice monoclonal anti-AFP antibody conjugate. that is coated on the conjugate pad. The mixture moves along the membrane by capillary action and reacts with mice monoclonal anti-AFP antibody that is coated on the test region. If AFP is present, the result is the formation of a colored band in the test region. The gold conjugate will continue to migrate until is captured in the control region by immobilized goat anti-mouse IgG antibody and aggregating in a red line, which indicates the validity of the test
Test PROCEDURE
1. Bring all materials and specimens to room temperature.
2. Remove the test card from the sealed foil pouch.
3. Place the transfer pipette in the specimen and depress the bulb to withdraw a sample.
4. Hold the pipette in a vertical position over the sample well of the test card and deliver 70-100 ml of sample into the sample well.
5. Read the result at 10 minutes.
Note: Results after 20 minutes may not be accurate
Specifications:
