Knowledge of vacutainer tubes color code - Healthaw Medical Limited

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Knowledge of vacutainer tubes color code

1, Green Cap: tube containing Lithium Heparin or Sodium Heparin, heparin sulfate is a group containing the mucopolysaccharide with a strong negative charge, with enhance the inactivation of antithrombin Ⅲ the role of serine proteases, thereby preventing the thrombin formation and prevent platelet aggregation, such as the role of a wide range of anticoagulation. Heparin tubes are generally used for biochemical and blood flow change detection is the best choice for electrolyte testing. Because heparin can cause leukocyte aggregation, it can not be used for white blood cell count and classification.

2, Red Cap: without additive or Clot Activator, tube wall evenly coated with silicon dioxide to prevent the hanging wall and at the same time added a coagulant, coagulant can activate plasminogen, so that is not soluble fibrin into soluble fibrin polymer, and then form a stable The fibrin clot. Generally used for hepatitis C virus, thyroid function, drug testing, AIDS testing, tumor markers, such as serological testing.

3, Lavender Cap: tube containing ethylenediaminetetraacetic acid and its salts (EDTA K2 or K3), it is a multi-amino carboxylic acid, can effectively chelate calcium in the blood, chelation of calcium from the reaction point will shift go, and to prevent and terminate endogenous or exogenous coagulation process, in order to prevent blood coagulation, in comparison with other anticoagulants, the agglutination of blood cells and blood cells was less affected, they usually use EDTA salt (2K , 3K, 2Na) as anticoagulant. Blood cell count and classification for general hematology examination, can not be used for coagulation, trace elements and PCR examination.

4, Blue Cap: tube containing sodium citrate, sodium citrate in the blood through the chelation of calcium ions into the role of anticoagulation, the National Committee for Clinical Laboratory Standards (NCCLS) recommendation of 3.2% or 3.8%, anticoagulant 1:9 ratio and blood, mainly for fibrinolytic system (prothrombin time, thrombin time, activated partial thromboplastin time, fibrinogen) detection. Please note that when blood taken enough blood to eliminate the impact of dead space tube the accuracy of the test results. Blood immediately after mixing 5-8 times upside down.

5, Black Cap: tube containing sodium citrate, sodium citrate concentration of 3.2% (0.109mol / L) and 3.8% of the anticoagulant with the blood volume ratio is 1:4, the general as a result of ESR detection, When the ratio is too high anticoagulant, the blood diluted, can accelerate the sedimentation rate.

6, Yellow Cap: tube containing gel & clot actiator, conventional serum test (clinical biochemistry, immunology tests, radioimmunoassay tests, etc.) coagulant levels, including the separation of inert plastic

7, Gray Cap: plasma glucose tolerance test experiments sodium fluoride / potassium oxalate or sodium heparin

8, separation of white cells (cellular immunity, DNA analysis, flow cytometry experiments) gel cell separation, cell maintenance fluid

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